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After fixation, cells were permeabilized in 0.2% Triton X-100 solution in PBS for 5 min

by polynucleotide reassembly &/or polynucleotide site- saturation mutagenesis)

Cord blood monocyte-derived inflammatory cytokines suppress il-2 and induce nonclassic t(h)2-type immunity associated with development of food allergy